Research Reports - Cerebellar gene expression following human traumatic brain injury (TBI)

J Neurotrauma. 2012 Oct 2

Staffa K, Ondruschka B, Franke H, Dressler J

Gene expression of specific brain biomarkers offers the possibility to shed light
on the diffi-cult molecular pathways of traumatic brain injury (TBI) and may be
useful to estimate the age of trauma. Gene expression rates of cerebellar
injuries are not yet sufficiently established. In 12 cases of TBI including a
pathological change in cerebellum (mean age 42 years) with known survival times
ranging from an immediate death up to 96 hours, brain tissue samples from
different brain regions were analyzed with real-time PCR for expression of
Caspase-3, TrkB, S100B and GFAP mRNA. The pH was measured to gain information
about a possible correlation to RNA degradation. For comparison corresponding
brain regions were arranged from control samples died from sudden death. We found
a correlation between pH and the degradation of RNA in samples from the
contra-lateral site, where the samples with degraded RNA have a lower pH (p <
0.05). For short sur-vival times, the expression changes of Caspase-3 (p < 0.05)
and also the expression changes of TrkB (p < 0.1) in the cerebellum show a
significant increase compared to the controls. The cerebellar gene expression
changes seem to occur much faster and stronger compared to the other investigated
regions, in particular the cerebral trauma site. These findings could make the
cerebellum to an important target to study the expression changes after TBI.

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