Research Reports - Cerebellar gene expression following traumatic brain injury

J Neurotrauma. 2012 Nov 20;29(17):2716-21

Staffa K, Ondruschka B, Franke H, Dreßler J

Abstract Gene expression of specific brain biomarkers offers the possibility of
shedding light on the difficult molecular pathways of traumatic brain injury
(TBI) and may be useful to estimate the age of trauma. Gene expression rates of
cerebellar injuries are not yet sufficiently established. In 12 cases (mean age
42 years) of TBI including a pathological change in cerebellum (with known
survival times ranging from immediate death to 96 h), brain tissue samples from
different brain regions were analyzed with real-time polymerase chain reaction
(PCR) for expression of caspase-3, tyrosine kinase receptor B (TrkB), S100B, and
glial fibrillary acidic protein (GFAP) mRNA. The pH was measured to gain
information about a possible correlation to RNA degradation. For comparison,
corresponding brain regions were arranged from control samples of subjects that
died from sudden death. We found a correlation between pH and the degradation of
RNA in samples from the contralateral site, where the samples with degraded RNA
have a lower pH (p<0.05). For short survival times, the expression changes of
caspase-3 (p<0.05) and the expression changes of TrkB (p<0.1) in the cerebellum
show a significant increase compared to the controls. The cerebellar gene
expression changes seem to occur much faster and stronger compared to the other
investigated regions, in particular the cerebral trauma site. These findings
could make the cerebellum an important target area to study the expression
changes after TBI.

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